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In urine, O-dealkyl and N-deethyl-O-dealkyl metabolites and glucuronides ended up predominant. Isotonitazene and metonitazene O-dealkyl metabolites have been a similar molecules and may also be probable generated in the metabolization of structural analogues including etonitazene, protonitazene, or butonitazene, whose only difference from isotonita